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CURRENT STATE OF THE FIELD...
LACK OF HORMONE MEASUREMENTS IN RODENT STUDIES
Measuring steroids are difficult - particularly in rodents due to low steroid concentrations for most analytes.
Limitations in sensitive and precise methodologies have limited the measurement of steroids in rodent experiments
This has resulted in the use of supraphysiological doses of steroids being administered, particularly estradiol, in basic science studies.
How do we move the field forward? Regular and accurate measurements of steroids. This is what we hope to accomplish.
THINGS TO CONSIDER....
SEX STEROID FLUCTUATIONS IN FEMALES
If examining sex steroids in females, have you accounted for the stage of the estrous cycle when collecting samples?
This is important as others, including ourselves, have shown significant changes in circulating and tissue sex steroids during the estrous cycle.
We have shown that tissue sex steroid content is typically higher on a per gram basis in tissue than in serum/plasma. You may want to think about measuring steroids in your tissue particularly if estradiol is your primary steroid of interest.
Sex Steroid Fluctuations.pdfSEX STEROID FLUCTUATIONS IN MALES
Collaborators are commonly shocked at how much androgen levels fluctuate in male rodents. Large day-to-day and intraday fluctuations (as much as 100x) in male rodents are normal.
Testosterone Fluctuations in Mice.pdfSAMPLE VOLUME
The more the better!
While our instrument sensitivity is state-of-the-art, limits in sample volume hinder our ability to measure hard to detect steroids like estradiol (E2). Contact Dr. Enos for guidance on this.
WHAT CAN WE MEASURE IN RODENTS?
WHAT WE LOOK FOR:
Aldosterone
Androstenedione
Corticosterone
11-Deoxycorticosterone
DHT
E1 (Estrone)
E2 (Estradiol)
E3 (Estriol)
Progesterone
Testosterone
T3 and Reverse T3 (Triiodothyronine) (Thyroid Hormone)
T4 (Thyroxine) (Thyroid Hormone)
DHEA-Sulfate
E1-Sulfate
E2-Sulfate
11-Ketotestosterone
WHAT WE CAN PROBE FOR ON REQUEST:
Cortisol
11-Deoxycortisol
17alpha-hydroxyprogesterone
Please note that detectability of a given steroid is dependent on treatment (e.g. hormone replacement therapy, gonadectomy, etc.) and sample volume. Steroids that are highly detectable are often detected regardless of treatment.
If there are additional steroids you are interested in measuring and they aren't on this list we can try and measure it for you - just let us know!
Please complete the sample inquiry form below and email Dr. Enos directly at enosr@email.sc.edu with questions regarding pricing:
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